Jay Keasling

Jay Keasling

Scientific Director

DTU BIOSUSTAIN
Novo Nordisk Foundation Center for Biosustainability

Technical University of Denmark

Kogle Allé 6

Building Cederhuset, room xx

2970 Hørsholm

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2019
 

A High-Throughput Mass Spectrometric Enzyme Activity Assay Enabling the Discovery of Cytochrome P450 Biocatalysts

de Rond, Tristan ; Gao, Jian ; Zargar, Amin ; de Raad, Markus ; Cunha, Jack ; Northen, Trent R. ; Keasling, Jay D.
in: ANGEWANDTE CHEMIE, vol: 58, issue: 30, pages: 10114-10119

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2019     |    DOI: https://doi.org/10.1002/anie.201901782

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A rapid methods development workflow for high-throughput quantitative proteomic applications

Chen, Yan ; Vu, Jonathan ; Thompson, Mitchell G. ; Sharpless, William A. ; Chan, Leanne Jade G. ; Gin, Jennifer W. ; Keasling, Jay D. ; Adams, Paul D. ; Petzold, Christopher J.
in: PLOS ONE, vol: 14, issue: 2

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2019     |    DOI: https://doi.org/10.1371/journal.pone.0211582

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Building a global alliance of biofoundries

Hillson, Nathan ; Caddick, Mark ; Cai, Yizhi ; Carrasco, Jose A. ; Chang, Matthew Wook ; Curach, Natalie C. ; Bell, David J. ; Le Feuvre, Rosalind ; Friedman, Douglas C. ; Fu, Xiongfei ; Gold, Nicholas D. ; Herrgård, Markus J. ; Holowko, Maciej B. ; Johnson, James R. ; Johnson, Richard A. ; Keasling, Jay D. ; Kitney, Richard I. ; Kondo, Akihiko ; Liu, Chenli ; Martin, Vincent J.J. ; Menolascina, Filippo ; Ogino, Chiaki ; Patron, Nicola J. ; Pavan, Marilene ; Poh, Chueh Loo ; Pretorius, Isak S. ; Rosser, Susan J. ; Scrutton, Nigel S. ; Storch, Marko ; Tekotte, Hille ; Travnik, Evelyn ; Vickers, Claudia E. ; Yew, Wen Shan ; Yuan, Yingjin ; Zhao, Huimin ; Freemont, Paul S.
in: Nature Communications, vol: 10

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2019     |    DOI: https://doi.org/10.1038/s41467-019-10079-2

 

Complete biosynthesis of cannabinoids and their unnatural analogues in yeast

Luo, Xiaozhou ; Reiter, Michael A. ; d’Espaux, Leo ; Wong, Jeff ; Denby, Charles M. ; Lechner, Anna ; Zhang, Yunfeng ; Grzybowski, Adrian T. ; Harth, Simon ; Lin, Weiyin ; Lee, Hyunsu ; Yu, Changhua ; Shin, John ; Deng, Kai ; Benites, Veronica T. ; Wang, George ; Baidoo, Edward E.K. ; Chen, Yan ; Dev, Ishaan ; Petzold, Christopher J. ; Keasling, Jay D.
in: Nature, vol: 567, issue: 7746, pages: 123-126

Type: Letter (Peer reviewed)

Status: Published     |    Year: 2019     |    DOI: https://doi.org/10.1038/s41586-019-0978-9

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Engineered Reversal of Function in Glycolytic Yeast Promoters

Rajkumar, Arun S. ; Özdemir, Emre ; Lis, Alicia V. ; Schneider, Konstantin ; Qin, Jiufu ; Jensen, Michael K. ; Keasling, Jay D.
in: ACS Synthetic Biology, vol: 8, issue: 6, pages: 1462-1468

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2019     |    DOI: https://doi.org/10.1021/acssynbio.9b00027

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Engineering glucose metabolism of escherichia coli under nitrogen starvation

Chubukov, Victor ; Desmarais, John James ; Wang, George ; Chan, Leanne Jade G. ; Baidoo, Edward E.K. ; Petzold, Christopher J. ; Keasling, Jay D. ; Mukhopadhyay, Aindrila
in: n p j Systems Biology and Applications, vol: 3, issue: 1

Type: Journal article (Peer reviewed)

Status: Accepted/In press     |    Year: 2019     |    DOI: https://doi.org/10.1038/npjsba.2016.35

 

Liquid chromatography and mass spectrometry analysis of isoprenoid intermediates in Escherichia coli

Baidoo, Edward E.K. ; Wang, George ; Joshua, Chijioke J. ; Benites, Veronica Teixeira ; Keasling, Jay D.
part of: Microbial Metabolomics, pages: 209-224, 2019, Humana Press, New York

Type: Book chapter (Peer reviewed)

Status: Published     |    Year: 2019     |    DOI: https://doi.org/10.1007/978-1-4939-8757-3_11

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Massively parallel fitness profiling reveals multiple novel enzymes in Pseudomonas putida lysine metabolism

Thompson, Mitchell G. ; Blake-Hedges, Jacquelyn M. ; Cruz-Morales, Pablo ; Barajas, Jesus F. ; Curran, Samuel C. ; Eiben, Christopher B. ; Harris, Nicholas C. ; Benites, Veronica T. ; Gin, Jennifer W. ; Sharpless, William A. ; Twigg, Frederick F. ; Skyrud, Will ; Krishna, Rohith N. ; Pereira, Jose Henrique ; Baidoo, Edward E.K. ; Petzold, Christopher J. ; Adams, Paul D. ; Arkin, Adam P. ; Deutschbauer, Adam M. ; Keasling, Jay D.
in: mBio, vol: 10, issue: 3

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2019     |    DOI: https://doi.org/10.1128/mBio.02577-18

 

Structural insights into dehydratase substrate selection for the borrelidin and fluvirucin polyketide synthases

Barajas, Jesus F. ; McAndrew, Ryan P. ; Thompson, Mitchell G. ; Backman, Tyler W.H. ; Pang, Bo ; de Rond, Tristan ; Pereira, Jose H. ; Benites, Veronica T. ; Martín, Héctor García ; Baidoo, Edward E.K. ; Hillson, Nathan J. ; Adams, Paul D. ; Keasling, Jay D.
in: Journal of Industrial Microbiology and Biotechnology

Type: Journal article (Peer reviewed)

Status: Accepted/In press     |    Year: 2019     |    DOI: https://doi.org/10.1007/s10295-019-02189-z

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Sustainable bioproduction of the blue pigment indigoidine: Expanding the range of heterologous products in R. toruloides to include non-ribosomal peptides

Wehrs, Maren ; Gladden, John M. ; Liu, Yuzhong ; Platz, Lukas ; Prahl, Jan Philip ; Moon, Jadie ; Papa, Gabriella ; Sundstrom, Eric ; Geiselman, Gina M. ; Tanjore, Deepti ; Keasling, Jay D. ; Pray, Todd R. ; Simmons, Blake A. ; Mukhopadhyay, Aindrila
in: Green Chemistry, vol: 21, issue: 12, pages: 3394-3406

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2019     |    DOI: https://doi.org/10.1039/c9gc00920e

 

Synthetic Biology for Fundamental Biochemical Discovery

Budin, Itay ; Keasling, Jay D.
in: Biochemistry, vol: 58, issue: 11, pages: 1464-1469

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2019     |    DOI: https://doi.org/10.1021/acs.biochem.8b00915

 

Technical Advances to Accelerate Modular Type I Polyketide Synthase Engineering towards a Retro-biosynthetic Platform

Pang, Bo ; Valencia, Luis E. ; Wang, Jessica ; Wan, Yao ; Lal, Ravi ; Zargar, Amin ; Keasling, Jay D.
in: Biotechnology and Bioprocess Engineering, vol: 24, issue: 3, pages: 413-423

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2019     |    DOI: https://doi.org/10.1007/s12257-019-0083-9

2018
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A combinatorial approach to synthetic transcription factor-promoter combinations for yeast strain engineering

Dossani, Zain Y. ; Apel, Amanda Reider ; Szmidt-Middleton, Heather ; Hillson, Nathan J. ; Deutsch, Samuel ; Keasling, Jay D. ; Mukhopadhyay, Aindrila
in: Yeast, vol: 35, issue: 3, pages: 273-280

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1002/yea.3292

 

An Orthogonal and pH-Tunable Sensor-Selector for Muconic Acid Biosynthesis in Yeast

Snoek, Tim ; Romero-Suarez, David ; Zhang, Jie ; Ambri, Francesca ; Skjoedt, Mette L. ; Sudarsan, Suresh ; Jensen, Michael K. ; Keasling, Jay D.
in: A C S Synthetic Biology, vol: 7, issue: 4, pages: 995-1003

Type: Letter (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1021/acssynbio.7b00439

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A Pseudomonas putida efflux pump acts on short-chain alcohols

Basler, Georg ; Thompson, Mitchell ; Tullman-Ercek, Danielle ; Keasling, Jay
in: Biotechnology for Biofuels, vol: 11, issue: 1

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1186/s13068-018-1133-9

 

Assembly and Multiplex Genome Integration of Metabolic Pathways in Yeast Using CasEMBLR

Jakočiūnas, Tadas ; Jensen, Emil D. ; Jensen, Michael Krogh ; Keasling, Jay D.
part of: Synthetic Metabolic Pathways, pages: 185-201, 2018

Type: Book chapter (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1007/978-1-4939-7295-1_12

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Biochemical Characterization of β-Amino Acid Incorporation in Fluvirucin B2 Biosynthesis

Barajas, Jesus F. ; Zargar, Amin ; Pang, Bo ; Benites, Veronica T. ; Gin, Jennifer ; Baidoo, Edward E. K. ; Petzold, Christopher J. ; Hillson, Nathan J. ; Keasling, Jay D.
in: Chembiochem, vol: 19, issue: 13, pages: 1391-1395

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1002/cbic.201800169

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CasPER, a method for directed evolution in genomic contexts using mutagenesis and CRISPR/Cas9

Jakočiūnas, Tadas ; Pedersen, Lasse E. ; Lis, Alicia V. ; Jensen, Michael K. ; Keasling, Jay D.
in: Metabolic Engineering, vol: 48, pages: 288-296

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1016/j.ymben.2018.07.001

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ClusterCAD: a computational platform for type I modular polyketide synthase design

Eng, Clara H. ; Backman, Tyler W. H. ; Bailey, Constance B. ; Magnan, Christophe ; Martin, Hector Garcia ; Katz, Leonard ; Baldi, Pierre ; Keasling, Jay D.
in: Nucleic acids research, vol: 46, issue: D1, pages: D509-D515

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1093/nar/gkx893

 

Commodity Chemicals From Engineered Modular Type I Polyketide Synthases

Yuzawa, Satoshi ; Zargar, Amin ; Pang, Bo ; Katz, Leonard ; Keasling, Jay D.
part of: Methods in Enzymology, pages: 393-415, 2018, Elsevier

Type: Book chapter (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1016/bs.mie.2018.04.027

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Constraining genome-scale models to represent the bow tie structure of metabolism for 13C metabolic flux analysis

Backman, Tyler W.H. ; Ando, David ; Singh, Jahnavi ; Keasling, Jay D. ; Martín, Héctor García
in: Metabolites, vol: 8, issue: 1

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.3390/metabo8010003

 

Cover Feature: Biochemical Characterization of β‐Amino Acid Incorporation in Fluvirucin B2 Biosynthesis (ChemBioChem 13/2018)

Barajas, Jesus F. ; Zargar, Amin ; Pang, Bo ; Benites, Veronica T. ; Gin, Jennifer ; Baidoo, Edward E. K. ; Petzold, Christopher J. ; Hillson, Nathan J. ; Keasling, Jay D.

Type: 2D/3D (physical products)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1002/cbic.201800299

 

Design, Engineering, and Characterization of Prokaryotic Ligand-Binding Transcriptional Activators as Biosensors in Yeast

Ambri, Francesca ; Snoek, Tim ; Skjødt, Mette Louise ; Jensen, Michael Krogh ; Keasling, Jay D.
part of: Synthetic Metabolic Pathways, pages: 269-290, 2018, Springer

Type: Book chapter (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1007/978-1-4939-7295-1_17

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Discovery of enzymes for toluene synthesis from anoxic microbial communities

Beller, Harry R. ; Rodrigues, Andria V. ; Zargar, Kamrun ; Wu, Yu-Wei ; Saini, Avneesh K. ; Saville, Renee M. ; Pereira, Jose H. ; Adams, Paul D. ; Tringe, Susannah G. ; Petzold, Christopher J. ; Keasling, Jay D.
in: Nature Chemical Biology, vol: 14, pages: 451-457

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1038/s41589-018-0017-4

 

Engineered Production of Short-Chain Acyl-Coenzyme A Esters in Saccharomyces cerevisiae

Krink-Koutsoubelis, Nicolas ; Loechner, Anne C. ; Lechner, Anna ; Link, Hannes ; Denby, Charles M. ; Vögeli, Bastian ; Erb, Tobias J. ; Yuzawa, Satoshi ; Jakociunas, Tadas ; Katz, Leonard ; Jensen, Michael K. ; Sourjik, Victor ; Keasling, Jay D
in: A C S Synthetic Biology, vol: 7, issue: 4, pages: 1105-1115

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1021/acssynbio.7b00466

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Engineering β-oxidation in Yarrowia lipolytica for methyl ketone production

Hanko, Erik K. R. ; Denby, Charles M. ; Nogué, Violeta Sànchez I ; Lin, Weiyin ; Ramirez, Kelsey J. ; Singer, Christine A. ; Beckham, Gregg T. ; Keasling, Jay D.
in: Metabolic Engineering, vol: 48, pages: 52-62

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1016/j.ymben.2018.05.018

 

De novo DNA synthesis using polymerasenucleotide conjugates

Palluk, Sebastian ; Arlow, Daniel H ; De Rond, Tristan ; Barthel, Sebastian ; Kang, Justine S ; Bector, Rathin ; Baghdassarian, Hratch M ; Truong, Alisa N ; Kim, Peter W ; Singh, Anup K. ; Hillson, Nathan J ; Keasling, Jay D
in: Nature Biotechnology, vol: 36, issue: 7, pages: 645-650

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1038/nbt.4173

 

Improving methyl ketone production in Escherichia coli by heterologous expression of NADH-dependent FabG

Goh, Ee Been ; Chen, Yan ; Petzold, Christopher J. ; Keasling, Jay D. ; Beller, Harry R.
in: Biotechnology and Bioengineering, vol: 115, issue: 5, pages: 1161-1172

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1002/bit.26558

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Industrial brewing yeast engineered for the production of primary flavor determinants in hopped beer

Denby, Charles M. ; Li, Rachel A. ; Vu, Van T. ; Costello, Zak ; Lin, Weiyin ; Chan, Leanne Jade G. ; Williams, Joseph ; Donaldson, Bryan ; Bamforth, Charles W. ; Petzold, Christopher J. ; Scheller, Henrik V. ; Martin, Hector Garcia ; Keasling, Jay D.
in: Nature Communications, vol: 9

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1038/s41467-018-03293-x

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Integrated analysis of isopentenyl pyrophosphate (IPP) toxicity in isoprenoid-producing Escherichia coli

George, Kevin W ; Thompson, Mitchell ; Kim, Joonhoon ; Baidoo, Edward E K ; Wang, George ; Benites, Veronica Teixeira ; Petzold, Christopher J ; Chan, Leanne Jade G ; Yilmaz, Suzan ; Turhanen, Petri ; Adams, Paul D ; Keasling, Jay D ; Lee, Taek Soon
in: Metabolic Engineering, vol: 47, pages: 60-72

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1016/j.ymben.2018.03.004

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Isolation and characterization of novel mutations in the pSC101 origin that increase copy number

Thompson, Mitchell G. ; Sedaghatian, Nima ; Barajas, Jesus F. ; Wehrs, Maren ; Bailey, Constance B. ; Kaplan, Nurgul ; Hillson, Nathan J. ; Mukhopadhyay, Aindrila ; Keasling, Jay D.
in: Scientific Reports, vol: 8

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1038/s41598-018-20016-w

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Modular 5′-UTR hexamers for context-independent tuning of protein expression in eukaryotes

Petersen, Søren D. ; Zhang, Jie ; Lee, Jae S. ; Jakočiūnas, Tadas ; Grav, Lise M. ; Kildegaard, Helene F. ; Keasling, Jay D. ; Jensen, Michael K.
in: Nucleic acids research, vol: 46, issue: 21

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1093/nar/gky734

 

Polyketide synthases as a platform for chemical product design

Zargar, Amin ; Barajas, Jesus F. ; Lal, Ravi ; Keasling, Jay D.
in: AIChE Journal, vol: 64, issue: 12, pages: 4201-4207

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1002/aic.16351

 

Probing the Flexibility of an Iterative Modular Polyketide Synthase with Non-Native Substrates in Vitro

Curran, Samuel C. ; Hagen, Andrew ; Poust, Sean ; Chan, Leanne Lade G. ; Garabedian, Brett M. ; de Rond, Tristan ; Baluyot, Marian-Joy ; Vu, Jonathan T. ; Lau, Andrew K. ; Yuzawa, Satoshi ; Petzold, Christopher J. ; Katz, Leonard ; Keasling, Jay D.
in: A C S Chemical Biology, vol: 13, issue: 8, pages: 2261-2268

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1021/acschembio.8b00422

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Production efficiency of the bacterial non-ribosomal peptide indigoidine relies on the respiratory metabolic state in S. cerevisiae

Wehrs, Maren ; Prahl, Jan-Philip ; Moon, Jadie ; Li, Yuchen ; Tanjore, Deepti ; Keasling, Jay D. ; Pray, Todd ; Mukhopadhyay, Aindrila
in: Microbial Cell Factories, vol: 17, issue: 1

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1186/s12934-018-1045-1

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Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli

Liu, Chun-Li ; Tian, Tian ; Alonso-Gutierrez, Jorge ; Garabedian, Brett ; Wang, Shuai ; Baidoo, Edward E. K. ; Benites, Veronica ; Chen, Yan ; Petzold, Christopher J. ; Adams, Paul D. ; Keasling, Jay D. ; Tan, Tianwei ; Lee, Taek Soon
in: Biotechnology for Biofuels, vol: 11

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1186/s13068-018-1272-z

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Short-chain ketone production by engineered polyketide synthases in Streptomyces albus

Yuzawa, Satoshi ; Mirsiaghi, Mona ; Jocic, Renee ; Fujii, Tatsuya ; Masson, Fabrice ; Benites, Veronica T. ; Baidoo, Edward E. K. ; Sundstrom, Eric ; Tanjore, Deepti ; Pray, Todd R. ; George, Anthe ; Davis, Ryan W. ; Gladden, John M. ; Simmons, Blake A. ; Katz, Leonard ; Keasling, Jay D.
in: Nature Communications, vol: 9

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1038/s41467-018-07040-0

 

Synthetic biology of polyketide synthases

Yuzawa, Satoshi ; Backman, Tyler W.H. ; Keasling, Jay D. ; Katz, Leonard
in: Journal of Industrial Microbiology and Biotechnology, vol: 45, issue: 7, pages: 621-633

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1007/s10295-018-2021-9

 

Synthetic Metabolic Pathways

Jensen, Michael Krogh ; Keasling, Jay

Type: Book (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1007/978-1-4939-7295-1

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Toward industrial production of isoprenoids in Escherichia coli: Lessons learned from CRISPR-Cas9 based optimization of a chromosomally integrated mevalonate pathway

Alonso-Gutierrez, Jorge ; Koma, Daisuke ; Hu, Qijun ; Yang, Yuchen ; Chan, Leanne J.G. ; Petzold, Christopher J. ; Adams, Paul D. ; Vickers, Claudia E. ; Nielsen, Lars K. ; Keasling, Jay D. ; Lee, Taek S.
in: Biotechnology and Bioengineering, vol: 115, issue: 4, pages: 1000-1013

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1002/bit.26530

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Use of prokaryotic transcriptional activators as metabolite biosensors in eukaryotic cells

Jensen, Michael Krogh ; Keasling, Jay ; Skjødt, Mette Louise ; Snoek, Tim
Patent no.: WO2018007526 , Date: 2018-01-11

Type: Patent

Status: Published     |    Year: 2018

 

Viscous control of cellular respiration by membrane lipid composition

Budin, Itay ; Rond, Tristan de ; Chen, Yan ; Chan, Leanne Jade G. ; Petzold, Christopher J. ; Keasling, Jay D.
in: Science, vol: 362, issue: 6419, pages: 1186–1189

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2018     |    DOI: https://doi.org/10.1126/science.aat7925

2017
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A Cas9-based toolkit to program gene expression in Saccharomyces cerevisiae

Apel, Amanda Reider ; d'Espaux, Leo ; Wehrs, Maren ; Sachs, Daniel ; Li, Rachel A. ; Tong, Gary J. ; Garber, Megan ; Nnadi, Oge ; Zhuang, William ; Hillson, Nathan J. ; Keasling, Jay D. ; Mukhopadhyay, Aindrila
in: Nucleic acids research, vol: 45, issue: 1, pages: 496-508

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1093/nar/gkw1023

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Application of an Acyl-CoA Ligase from Streptomyces aizunensis for Lactam Biosynthesis

Zhang, Jingwei ; Barajas, Jesus F. ; Burdu, Mehmet ; Wang, George ; Baidoo, Edward E. ; Keasling, Jay D
in: A C S Synthetic Biology, vol: 6, issue: 5, pages: 884-890

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1021/acssynbio.6b00372

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Autonomous control of metabolic state by a quorum sensing (QS)-mediated regulator for bisabolene production in engineered E. coli

Kim, Eun-Mi ; Min Woo, Han ; Tian, Tian ; Yilmaz, Suzan ; Javidpour, Pouya ; Keasling, Jay D. ; Soon Lee, Taek
in: Metabolic Engineering, vol: 44, pages: 325-336

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1016/j.ymben.2017.11.004

 

Base-Catalyzed Depolymerization of Solid Lignin-Rich Streams Enables Microbial Conversion

Rodriguez, Alberto ; Salvachúa, Davinia ; Katahira, Rui ; Black, Brenna A. ; Cleveland, Nicholas S. ; Reed, Michelle ; Smith, Holly ; Baidoo, Edward E.K. ; Keasling, Jay D. ; Simmons, Blake A. ; Beckham, Gregg T. ; Gladden, John M.
in: A C S Sustainable Chemistry & Engineering, vol: 5, issue: 9, pages: 8171-8180

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1021/acssuschemeng.7b01818

 

Bio-based production of fuels and industrial chemicals by repurposing antibiotic-producing type I modular polyketide synthases: opportunities and challenges

Yuzawa, Satoshi ; Keasling, Jay D. ; Katz, Leonard
in: The Journal of Antibiotics, vol: 70, pages: 378-385

Type: Review (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1038/ja.2016.136

 

Comprehensive in Vitro Analysis of Acyltransferase Domain Exchanges in Modular Polyketide Synthases and Its Application for Short-Chain Ketone Production

Yuzawa, Satoshi ; Deng, Kai ; Wang, George ; Baidoo, Edward E. K. ; Northen, Trent R. ; Adams, Paul D. ; Katz, Leonard ; Keasling, Jay
in: A C S Synthetic Biology, vol: 6, issue: 1, pages: 139-147

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1021/acssynbio.6b00176

 

Cyanobacterial carbon metabolism: Fluxome plasticity and oxygen dependence

Wan, Ni ; Delorenzo, Drew M. ; He, Lian ; You, Le ; Immethun, Cheryl M. ; Wang, George ; Baidoo, Edward E. K. ; Hollinshead, Whitney D. ; Keasling, Jay D. ; Moon, Tae Seok ; Tang, Yinjie J.
in: Biotechnology and Bioengineering, vol: 114, issue: 7, pages: 1593-1602

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1002/bit.26287

 

Deciphering flux adjustments of engineered E. coli cells during fermentation with changing growth conditions

He, Lian ; Xiu, Yu ; Jones, J. Andrew ; Baidoo, Edward E. K. ; Keasling, Jay D. ; Tang, Yinjie J ; Koffas, Mattheos A. G.
in: Metabolic Engineering, vol: 39, pages: 247–256

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1016/j.ymben.2016.12.008

 

Development of an integrated approach for α-pinene recovery and sugar production from loblolly pine using ionic liquids

Papa, Gabriella ; Kirby, James ; Murthy Konda, N. V. S. N. ; Tran, Kim ; Singh, Seema ; Keasling, Jay D. ; Peter, Gary F. ; Simmons, Blake A.
in: Green Chemistry, vol: 19, pages: 1117-1127

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1039/c6gc02637k

 

Development of a Transcription Factor-Based Lactam Biosensor

Zhang, Jingwei ; Barajas, Jesus F. ; Burdu, Mehmet ; Ruegg, Thomas L. ; Dias, Bryton ; Keasling, Jay
in: A C S Synthetic Biology, vol: 6, issue: 3, pages: 439-445

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1021/acssynbio.6b00136

 

Development of Next Generation Synthetic Biology Tools for Use in Streptomyces venezuelae

Phelan, Ryan M. ; Sachs, Daniel ; Petkiewicz, Shayne J. ; Barajas, Jesus F. ; Blake-Hedges, Jacquelyn M. ; Thompson, Mitchell G. ; Apel, Amanda Reider ; Rasor, Blake J. ; Katz, Leonard ; Keasling, Jay D.
in: A C S Synthetic Biology, vol: 6, issue: 1, pages: 159-166

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1021/acssynbio.6b00202

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Engineered polyketides: Synergy between protein and host level engineering

Barajas, Jesus F. ; Blake-Hedges, Jacquelyn M. ; Bailey, Constance B. ; Curran, Samuel ; Keasling, Jay D.
in: Synthetic and Systems Biotechnology, vol: 2, issue: 3, pages: 147-166

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1016/j.synbio.2017.08.005

 

Engineering high-level production of fatty alcohols by Saccharomyces cerevisiae from lignocellulosic feedstocks

d'Espaux, Leo ; Ghosh, Amit ; Runguphan, Weerawat ; Wehrs, Maren ; Xu, Feng ; Konzock, Oliver ; Dev, Ishaan ; Nhan, Melissa ; Gin, Jennifer ; Apel, Amanda Reider ; Petzold, Christopher J. ; Singh, Seema ; Simmons, Blake A. ; Mukhopadhyay, Aindrila ; Martín, Hector García ; Keasling, Jay D.
in: Metabolic Engineering, vol: 42, pages: 115-125

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1016/j.ymben.2017.06.004

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Erratum to: The JBEI quantitative metabolic modeling library (jQMM): a python library for modeling microbial metabolism

Birkel, Garrett W. ; Ghosh, Amit ; Kumar, Vinay S. ; Weaver, Daniel ; Ando, David ; Backman, Tyler W. H. ; Arkin, Adam Paul ; Keasling, Jay ; Martín, Héctor García
in: B M C Bioinformatics, vol: 18

Type: Comment/debate (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1186/s12859-017-1631-y

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Flux-Enabled Exploration of the Role of Sip1 in galactose yeast metabolism

Shymansky, Christopher M. ; Wang, George ; Baidoo, Edward E. K. ; Gin, Jennifer ; Apel, Amanda Reider ; Mukhopadhyay, Aindrila ; Martin, Hector Garcia ; Keasling, Jay
in: Frontiers in Bioengineering and Biotechnology, vol: 5

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.3389/fbioe.2017.00031

 

Genetically-encoded biosensors for yeast cell factory optimization

Ambri, Francesca ; Jensen, Michael K. ; Keasling, Jay D.
part of: Synthetic Biology Conference (SEED 2017) Engineering, Evolution, and Design , pages: 193-194, 2018, AIChE, American Institute of Chemical Engineers

Type: Conference abstract in proceedings (Peer reviewed)

Status: Published     |    Year: 2017

 

Heterologous Gene Expression of N-Terminally Truncated Variants of LipPks1 Suggests a Functionally Critical Structural Motif in the N-terminus of Modular Polyketide Synthase

Yuzawa, Satoshi ; Bailey, Constance B. ; Fujii, Tatsu A. ; Jocic, Renee ; Barajas, Jesus F. ; Benites, Veronica T. ; Baidoo, Edward E. K. ; Chen, Yan ; Petzold, Christopher J. ; Katz, Leonard ; Keasling, Jay D.
in: A C S Chemical Biology, vol: 12, issue: 11, pages: 2725-2729

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1021/acschembio.7b00714

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High-throughput enzyme screening platform for the IPP-bypass mevalonate pathway for isopentenol production

Kang, Aram ; Meadows, Corey W. ; Canu, Nicolas ; Keasling, Jay D. ; Lee, Taek Soon
in: Metabolic Engineering, vol: 41, pages: 125-134

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1016/j.ymben.2017.03.010

 

Intracellular cellobiose metabolism and its applications in lignocellulose-based biorefineries

Parisutham, Vinuselvi ; Chandran, Sathesh-Prabu ; Mukhopadhyay, Aindrila ; Lee, Sung Kuk ; Keasling, Jay D
in: Bioresource Technology, vol: 239, pages: 496-506

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1016/j.biortech.2017.05.001

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Leveraging microbial biosynthetic pathways for the generation of 'drop-in' biofuels

Zargar, Amin ; Bailey, Constance B. ; Haushalter, Robert W. ; Eiben, Christopher B. ; Katz, Leonard ; Keasling, Jay D.
in: Current Opinion in Biotechnology, vol: 45, pages: 156-163

Type: Review (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1016/j.copbio.2017.03.004

 

Lipid engineering reveals regulatory roles for membrane fluidity in yeast flocculation and oxygen-limited growth

Degreif, Daniel ; de Rond, Tristan ; Bertl, Adam ; Keasling, Jay D ; Budin, Itay
in: Metabolic Engineering, vol: 41, pages: 46-56

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1016/j.ymben.2017.03.002

 

OpenMSI Arrayed Analysis Toolkit: Analyzing Spatially Defined Samples Using Mass Spectrometry Imaging

de Raad, Markus ; de Rond, Tristan ; Rübel, Oliver ; Keasling, Jay ; Northen, Trent R. ; Bowen, Benjamin P.
in: Analytical chemistry, vol: 89, issue: 11, pages: 5818-5823

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1021/acs.analchem.6b05004

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Oxidative cyclization of prodigiosin by an alkylglycerol monooxygenase-like enzyme

de Rond, Tristan ; Stow, Parker ; Eigl, Ian ; Johnson, Rebecca E. ; Chan, Leanne Jade G. ; Goyal, Garima ; Baidoo, Edward E. K. ; Hillson, Nathan J. ; Petzold, Christopher J. ; Sarpong, Richmond ; Keasling, Jay D.
in: Nature Chemical Biology, vol: 13, issue: 11, pages: 1155-1157

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1038/nchembio.2471

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Production of jet fuel precursor monoterpenoids from engineered Escherichia coli

Mendez-Perez, Daniel ; Alonso-Gutierrez, Jorge ; Hu, Qijun ; Molinas, Margaux ; Baidoo, Edward E. K. ; Wang, George ; Chan, Leanne J. G. ; Adams, Paul D. ; Petzold, Christopher J. ; Keasling, Jay D. ; Lee, Taek S.
in: Biotechnology and Bioengineering, vol: 114, issue: 8, pages: 1703-1712

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1002/bit.26296

 

System-level perturbations of cell metabolism using CRISPR/Cas9

Jakociunas, Tadas ; Jensen, Michael Krogh ; Keasling, Jay
in: Current Opinion in Biotechnology, vol: 46, pages: 134-140

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1016/j.copbio.2017.03.014

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The Experiment Data Depot: A Web-Based Software Tool for Biological Experimental Data Storage, Sharing, and Visualization

Morell, William C. ; Birkel, Garrett W. ; Forrer, Mark ; Lopez, Teresa ; Backman, Tyler W. H. ; Dussault, Michael ; Petzold, Christpoher J. ; Baidoo, Edward E. K. ; Costello, Zak ; Ando, David ; Alonso-Gutierrez, Jorge ; George, Kevin W. ; Mukhopadhyay, Aindrila ; Vaino, Ian ; Keasling, Jay ; Adams, Paul D. ; Hillson, Nathan J. ; Garcia Martin, Hector
in: A C S Synthetic Biology, vol: 6, issue: 12, pages: 2248-2259

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1021/acssynbio.7b00204

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The JBEI quantitative metabolic modeling library (jQMM): a python library for modeling microbial metabolism

Birkel, Garrett W. ; Ghosh, Amit ; Kumar, Vinay S. ; Weaver, Daniel ; Ando, David ; Backman, Tyler W. H. ; Arkin, Adam Paul ; Keasling, Jay D. ; Martin, Hector Garcia
in: B M C Bioinformatics, vol: 18

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1186/s12859-017-1615-y

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Transcriptional reprogramming in yeast using dCas9 and combinatorial gRNA strategies

Damgaard Jensen, Emil ; Ferreira, Raphael ; Jakociunas, Tadas ; Arsovska, Dushica ; Zhang, Jie ; Ding, Ling ; Smith, Justin D ; David, Florian ; Nielsen, Jens ; Jensen, Michael Krogh ; Keasling, Jay D
in: Microbial Cell Factories, vol: 16

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2017     |    DOI: https://doi.org/10.1186/s12934-017-0664-2

2016
 

CRISPR/Cas9 advances engineering of microbial cell factories

Jakociunas, Tadas ; Jensen, Michael Krogh ; Keasling, Jay D.
in: Metabolic Engineering, vol: 34, pages: 44-59

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2016     |    DOI: https://doi.org/10.1016/j.ymben.2015.12.003

  PDF

EasyClone-MarkerFree

Fabre, Mathew Malcolm Jessop ; Jakociunas, Tadas ; Stovicek, Vratislav ; Dai, Z. ; Jensen, Michael Krogh ; Keasling, Jay ; Borodina, Irina
in: Biotechnology Journal, vol: 11, issue: 8, pages: 1110-1117

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2016     |    DOI: https://doi.org/10.1002/biot.201600147

 

Engineering a functional 1-deoxy-D-xylulose 5-phosphate (DXP) pathway in Saccharomyces cerevisiae

Kirby, James ; Dietzel, Kevin L. ; Wichmann, Gale ; Chan, Rossana ; Antipov, Eugene ; Moss, Nathan ; Baidoo, Edward E.K. ; Jackson, Peter ; Gaucher, Sara P. ; Gottlieb, Shayin ; LaBarge, Jeremy ; Mahatdejkul, Tina ; Hawkins, Kristy M. ; Muley, Sheela ; Newman, Jack D. ; Liu, Pinghua ; Keasling, Jay ; Zhao, Lishan
in: Metabolic Engineering, vol: 38, pages: 494-503

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2016     |    DOI: https://doi.org/10.1016/j.ymben.2016.10.017

 

Engineering an NADPH/NADPRedox Biosensor in Yeast

Zhang, Jie ; Sonnenschein, Nikolaus ; Pihl, Thomas Peter Boye ; Pedersen, Kasper Rud ; Jensen, Michael Krogh ; Keasling, Jay
in: A C S Synthetic Biology, vol: 5, issue: 12, pages: 1546–1556

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2016     |    DOI: https://doi.org/10.1021/acssynbio.6b00135

 

Engineering Bacteria to Catabolize the Carbonaceous Component of Sarin: Teaching E. coli to Eat Isopropanol

Brown, Margaret E. ; Mukhopadhyay, Aindrila ; Keasling, Jay D.
in: A C S Synthetic Biology, vol: 5, issue: 12, pages: 1485-1496

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2016     |    DOI: https://doi.org/10.1021/acssynbio.6b00115

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Engineering Cellular Metabolism

Nielsen, Jens ; Keasling, Jay
in: CELL, vol: 164, issue: 6, pages: 1185-1197

Type: Review (Peer reviewed)

Status: Published     |    Year: 2016     |    DOI: https://doi.org/10.1016/j.cell.2016.02.004

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Engineering of synthetic, stress-responsive yeast promoters

Rajkumar, Arun Stephen ; Liu, Guodong ; Bergenholm, David ; Arsovska, Dushica ; Kristensen, Mette ; Nielsen, Jens ; Jensen, Michael Krogh ; Keasling, Jay
in: Nucleic acids research, vol: 44, issue: 17

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2016     |    DOI: https://doi.org/10.1093/nar/gkw553

 

Engineering prokaryotic transcriptional activators as metabolite biosensors in yeast

Skjødt, Mette Louise ; Snoek, Tim ; Kildegaard, Kanchana Rueksomtawin ; Arsovska, Dushica ; Eichenberger, M. ; Goedecke, T. J. ; Rajkumar, Arun Stephen ; Zhang, Jie ; Kristensen, Mette ; Lehka, B. ; Siedler, Solvej ; Borodina, Irina ; Jensen, Michael Krogh ; Keasling, Jay
in: Nature Chemical Biology, vol: 12, issue: 11, pages: 951-958

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2016     |    DOI: https://doi.org/10.1038/nchembio.2177

  PDF

Enhanced fatty acid production in engineered chemolithoautotrophic bacteria using reduced sulfur compounds as energy sources

Beller, Harry R. ; Zhou, Peng ; Jewell, Talia N.M. ; Goh, Ee-Been ; Keasling, Jay
in: Metabolic Engineering Communications, vol: 3, pages: 211-215

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2016     |    DOI: https://doi.org/10.1016/j.meteno.2016.07.001

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Examining Escherichia coli glycolytic pathways, catabolite repression, and metabolite channeling using Δpfk mutants

Hollinshead, Whitney D. ; Rodriguez, Sarah ; Martin, Hector Garcia ; Wang, George ; Baidoo, Edward E. K. ; Sale, Kenneth L. ; Keasling, Jay ; Mukhopadhyay, Aindrila ; Tang, Yinjie J.
in: Biotechnology for Biofuels, vol: 9

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2016     |    DOI: https://doi.org/10.1186/s13068-016-0630-y

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Exploiting members of the BAHD acyltransferase family to synthesize multiple hydroxycinnamate and benzoate conjugates in yeast

Eudes, Aymerick ; Mouille, Maxence ; Robinson, David S. ; Benites, Veronica T. ; Wang, George ; Roux, Lucien ; Tsai, Yi-Lin ; Baidoo, Edward E. K. ; Chiu, Tsan-Yu ; Heazlewood, Joshua L. ; Scheller, Henrik V. ; Mukhopadhyay, Aindrila ; Keasling, Jay D. ; Deutsch, Samuel ; Loque, Dominique
in: Microbial Cell Factories, vol: 15

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2016     |    DOI: https://doi.org/10.1186/s12934-016-059-5

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In vitro Characterization of Phenylacetate Decarboxylase, a Novel Enzyme Catalyzing Toluene Biosynthesis in an Anaerobic Microbial Community

Zargar, K. ; Saville, R. ; Phelan, R. M. ; Tringe, S. G. ; Petzold, C. J. ; Keasling, Jay ; Beller, H. R.
in: Scientific Reports, vol: 6

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2016     |    DOI: https://doi.org/10.1038/srep31362

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13C Metabolic Flux Analysis for Systematic Metabolic Engineering of S. cerevisiae for Overproduction of Fatty Acids

Ghosh, Amit ; Ando, David ; Gin, Jennifer ; Runguphan, Weerawat ; Denby, Charles ; Wang, George ; Baidoo, Edward E. K. ; Shymansky, Chris ; Keasling, Jay ; García Martín, Héctor
in: Frontiers in Bioengineering and Biotechnology, vol: 4

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2016     |    DOI: https://doi.org/10.3389/fbioe.2016.00076

2015
 

Development of biosensors and their application in metabolic engineering

Zhang, Jie ; Jensen, Michael Krogh ; Keasling, Jay
in: Current Opinion in Chemical Biology, vol: 28

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2015     |    DOI: https://doi.org/10.1016/j.cbpa.2015.05.013

 

Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae

Jakociunas, Tadas ; Bonde, Ida ; Herrgard, Markus ; Harrison, Scott James ; Kristensen, Mette ; Pedersen, Lasse Ebdrup ; Jensen, Michael Krogh ; Keasling, Jay
in: Metabolic Engineering, vol: 28, pages: 213-222

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2015     |    DOI: https://doi.org/10.1016/j.ymben.2015.01.008

 

Recent applications of synthetic biology tools for yeast metabolic engineering

Jensen, Michael Krogh ; Keasling, Jay
in: FEMS Yeast Research, vol: 15, issue: 1

Type: Journal article (Peer reviewed)

Status: Published     |    Year: 2015     |    DOI: https://doi.org/10.1111/1567-1364.12185