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CRISPy-web is an online tool to design single guide RNAs (sgRNAs) for CRISPR applications with microbes. Unlike most other CRISPR design tools, CRISPy-web allows the user to upload custom/non-model organisms genome data to screen for off-target effects.

CRISPy-web can directly access antiSMASH results and use the antiSMASH-annotation to aid with the sgRNA design. CRISPy-web is based on CRISPy, a web-tool to find sgRNAs for Chinese hamster ovary (CHO-K1) cells.



Contact: Lasse Ebdrup Pedersen


Lasse Ebdrup Pedersen
Senior Researcher and Co-PI
DTU Biosustain
+45 21 12 73 21