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Seven-in-Seven is a newly developed method for fast generation of multiplex gene deletions in E. coli.

The method is based on lambda Red recombineering and flippase recombinase, and facilitates the generation of seven gene deletions in as little as seven days. The plasmid pSIJ8 and the strain SIJ488 are both available from Addgene. Other strains and plasmids generated in the study are available upon request from the authors. 

7 in 7 plasmid

References: Jensen SI, Lennen R, Herrgård MJ, Nielsen AT (2015). Seven gene deletions in seven days: Fast generation of Escherichia coli strains tolerant to acetate and osmotic stress. Scientific Reports 5: 17874. doi: 10.1038/srep17874

Contact: Prof. Alex T. Nielsen or Dr. Sheila I. Jensen


Alex Toftgaard Nielsen
DTU Biosustain
+45 21 79 89 06


Sheila Ingemann Jensen
Senior Researcher
DTU Biosustain
+45 24 92 18 30