CRISPy-web is an online tool to design single guide RNAs (sgRNAs) for CRISPR applications with microbes. Unlike most other CRISPR design tools, CRISPy-web allows the user to upload custom/non-model organisms genome data to screen for off-target effects.
CRISPy-web can directly access antiSMASH results and use the antiSMASH-annotation to aid with the sgRNA design. The initial version of CRISPy-web was based on CRISPy, a web-tool to find sgRNAs for Chinese hamster ovary (CHO-K1) cells.
CRISPy-web
References:
- Blin, K., Pedersen, L.E., Weber, T., Lee, S.Y., (2016) CRISPy-web: An online resource to design sgRNAs for CRISPR applications. Synth. Syst. Biotechnol., in press, doi: 10.1016/j.synbio.2016.1001.1003. (Open Access)
- Ronda, C., Pedersen, L.E., Hansen, H.G., Kallehauge, T.B., Betenbaugh, M.J., Nielsen, A.T., Kildegaard, H.F., (2014) Accelerating genome editing in CHO cells using CRISPR Cas9 and CRISPy, a web-based target finding tool. Biotechnol. Bioeng. 111, 1604-1616.
Contact: Tilmann Weber & Kai Blin