Pre-Pilot Plant

The Pre-Pilot Plant (PPP) is a translational research and development core section focused on bioprocess development and commercialisation of bio-based products from ideation to first prototype production.

The objective of the PPP is to attract and collaborate on both academic research, as well as industry-developed projects that fit the mission and vision of the Center.

The various operational units of the PPP are capable to accelerate and develop cell factories and bioprocesses to reach early commercial-level performance (TRL 5-7*) in a short period. We apply industrial standards, compliance, mindset and workflow in an integrated and agile design-build-test-learn environment. The international and interdisciplinary staff of the core consists of technicians, engineers and natural scientists, many with an industry career background.

We employ a broad range of innovative technologies, including automation, bioinformatics solutions on design, data capture and monitoring, In-Process-Control analytics, various bioreactors for strain optimisation (e.g. Sartorius ambr® 250 platform) as well as in vivo scale-down/-up to 150L volume. The downstream development is equipped with various unit operations from laboratory scale to pilot scale (up to 200kg). It includes solid-liquid separation, various filtration, liquid-liquid separation, evaporation, multiple chromatography columns, crystallisation etc. We rely on excellent partnerships of technology providers who have decades of production and manufacturing experience – The Pre-Pilot Plant provides scalable solutions, which can be implemented at industrial scale.

We operate with customer feedback, product specifications and “the-end-in-mind” under BSL1, non-GMP, no ATEX conditions.

Expanded Technology Readiness Level (TRL) Definitions for the Bioeconomy. Biofuelsdigest.com. Retrieved 7 November 2020
* Technology readiness levels (TRL); Extract from Part 19 - Commission Decision C(2014)4995" (PDF). ec.europa.eu. 2014. Retrieved 11 November 2019

 

 

 

Upstream Processing

Milica Randelovic
Lead Engineer Upstream, Pre-Pilot Plant
DTU Biosustain
+45 93 51 35 71

Downstream Processing

Minkle Jain
Lead Engineer Downstream
DTU Biosustain
+45 21 79 91 25

IPC Analytics

Gossa Garedew Wordofa
Lead Scientist, Biochemistry
DTU Biosustain
+45 93 51 12 85

Contact Pre-Pilot Plant

Andreas Worberg
Chief Commercial Officer
DTU Biosustain
+45 93 51 89 00

IPC Analytics (PPP) Instruments

3 HPLC
1 HPLC (dual)
1 Spectrophotometer
1 Total organic carbon and nitrogen measurement
1 Total Kjehdahl Nitrogen measurement
1 Digester
1 Melting- and boiling point determination

Upstream Processing Equipment

1 Microfluidic Microbioreactor (2.4mL)
24 Autmated Multi-Parallel Bioreactors (250mL)
12 Fermenter (1L)
2 Fermenter (5L)
2 Fermenter (30L)
1 Fermenter (100L)
1 Growth Profiler
1 Microbial Cell Counter
1 BioWelder

For a complete list of equipment click here

 

Downstream Processing Equipment - Lab scale

1 Rotary Evaporator
1 Membrane Filtration System
2 Jacketed Reactor (100mL and 1L)

Downstream Processing Equipment - Pilot scale

1 Disc-Stack Centrifuge
1 Rotary Evaporator
1 Spray Dryer
1 Homogenizer
1 Liquid-Liquid Extraction
1 Chromatography Column
1 Membrane Filtration System
1 Cleaning-In-Place Unit