Genome Engineering Unit

The Genome Engineering Unit is one of the four basic functional groups of the iLoop (Iterative Cell Factory Development Loop) Core section at DTU Biosustain.

This unit is responsible for management of iLoop projects, rapid construction of strains using various genome engineering techniques while also interfacing with the automated genome engineering unit for high-throughput strain construction, and conducting more specialized molecular biology and protein engineering workflows. 

The group is capable of constructing virtually any desired scarless genome modification or plasmid using the latest techniques integrated from the scientific sections at DTU Biosustain and beyond.

Methods used by the group for strain engineering in E. coli include homologous recombination with lambda Red recombinase, CRISPR/Cas9-mediated counterselection, P1 phage transduction, multiplex automated genome engineering (MAGE), USER cloning, and generation of mutagenesis libraries for protein engineering.

The group also develops strategies for growth coupling of production and develops platform strains with enhanced characteristics for use as cell factories. 

Genome engineering 1 

Figure 1 shows the tasks performed within the Genome Engineering Unit and interactions of the Unit inside and outside of iLoop.

Genome engineering figure 2

Figure 2 shows that the Unit has 90% efficiency of scarless integration of a 3 gene cassette using CRISPR/Cas9 technology.

Contact

Rebecca Lennen
Senior Researcher
DTU Biosustain
+45 21 12 66 39
http://www.biosustain.dtu.dk/english/research1/iloop-core-unit/genome-engineering-unit
23 SEPTEMBER 2017