Section for High-Throughput CHO Molecular Bioscience

The High-Throughput Molecular Bioscience group, situated at KTH in Stockholm, is advancing high-throughput technologies to facilitate a shift towards next generation CHO cell factories for production of protein therapeutics.

Chinese hamster ovary cells are the preferred cell line for bioproduction of biologics today. However, some products are not as easily produced as others. Here we explore the potential of bioproduction in CHO by making a high-throughput attempt to express all proteins naturally secreted in humans in CHO cells. The purpose is to learn more about the bottlenecks related to protein production and to feed this into the center activities related to editing of CHO cell lines to improve yield and quality of products. The final collection of all secreted proteins, the human secretome, is used in biological assays to find out more about their respective cellular function in relation to disease and human biology in a joint project together with industry and academia. 

As part of supporting the speeding up strain-development, we develop high throughput screening assays for increasing the screening capacity of cell libraries when developing industrial production strains. Here we develop and make use of droplet microfluidics, in order to encapsulate and screen individual CHO cells for their product yield and cellular state. 

Contact

Mathias Uhlén
Scientific Director
DTU Biosustain
http://www.biosustain.dtu.dk/english/Research1/Research-sections/HTMB
23 SEPTEMBER 2017