Helene FACS

Section for CHO Cell Line Engineering and Design

The CHO Cell Line Engineering and Design Section is addressing the need to rapidly obtain high yields and high quality of biopharmaceuticals produced in optimized CHO cells through genome-scale-based methodologies.

Watch this video to learn more about the work being done in this section:

Our main goal is to develop state-of-the-art genome editing technologies, omics data generation and high-throughput screening methods to improve CHO cell engineering efforts and facilitate identification of factors, that control growth, protein production and processing.

 Our main goals are to:

• Identify cellular factors, that enhance growth, productivity and protein quality through application of high-throughput screening technologies and systems biology data.

• Develop efficient and precise genome engineering tools to accelerate generation of CHO cell factories with improved clonal stability.

• Optimize engineering strategies for improved CHO cell factory design.

The section is located at the DTU Biosustain premises at DTU Lyngby and headed by professor Gyun Min Lee.

Main key publications:

Helene Faustrup Kildegaard, Deniz Baycin-Hizal, Nathan E. Lewis and Michael J. Betenbaugh. 2013. The emerging CHO systems biology era: harnessing the ‘omics revolution for biotechnology. Current Opinion in Biotechnology 24: 1102-1107. 

Carlotta Ronda, Lasse Ebdrup Pedersen, Henning Gram Hansen, Thomas Kallehauge, Michael J. Betenbaugh, Alex Toftgaard Nielsen and Helene Faustrup Kildegaard. 2014. Accelerating genome editing in CHO cells using CRISPR Cas9 and CRISPy, a web-based target finding tool. Biotechnology and Bioengineering 111: 1604-1616.

Jae Seong Lee, Thomas Beuchert Kallehauge, Lasse Ebdrup Pedersen and Helene Faustrup Kildegaard. 2015. Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway. Scientific Reports 5: article number 8572

Lise Marie Grav, Jae Seong Lee, Signe Gerling, Thomas Beuchert Kallehauge, Anders Holmgaard Hansen, Stefan Kol, Gyun Min Lee, Lasse Ebdrup Pedersen and Helene Faustrup Kildegaard. 2015. One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment. Biotechnology Journal 10 (9): 1446–1456

Jae Seong Lee, Lise Marie Grav, Nathan E. Lewis and Helene Faustrup Kildegaard. CRISPR/Cas9 mediated genome engineering of CHO cell factories: Application and perspectives. 2015. Invited review in Biotechnology Journal 10 (7): 979–994

Philipp N. Spahn, Anders H. Hansen, Henning Gram Hansen, Johnny Arnsdorf, Helene Faustrup Kildegaard and Nathan E. Lewis. A Markov chain model for N-linked protein glycosylation – towards low-parameter tool for model-driven glycoengineering. Metabolic Engineering (in press).

Contact

Helene Faustrup Kildegaard
Senior Researcher and Co-PI
DTU Biosustain
+45 20 12 46 29
http://www.biosustain.dtu.dk/english/Research1/Research-sections/CLED
23 SEPTEMBER 2017