Yeast Metabolic Engineering

We focus on metabolic engineering of yeast cell factories for biosustainable production of chemicals from renewable feedstocks. Our projects include both commodity chemicals, such as 3-hydroxypropionic acid for bioacrylics, and fine chemicals.

We develop genetic engineering tools, which facilitate iterative cycles of strain development, and work on general methodology for accelerated rational strain design, based on systems biology-level data (fluxome, transcriptome, metabolome, etc) and modeling.

GENETIC TOOLBOX for Saccharomyces cerevisiae

EasyClone vector set for simultaneous integration of multiple genes with an option of recycling selection markers can be ordered from BCCM Collection. [Order] [Jensen et al, 2014]

EasyClone 2.0 vector set with dominant selection markers is available from AddGene. [Order] [Manual] [Stovicek et al, 2015]

Borodina EasyClone kit vector map

EasyClone-MarkerFree vector set for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9 is available from AddGene. [Order] [Manual] [Jessop-Fabre & Jakočiūnas et al, 2016]

Complete kit for CRISPR-Cas9 mediated gene insertion into 11 different chromosomal loci without inserting markers.

EasyCloneMulti vector set for simultaneous and multiple genomic integrations in S. cerevisiae is available upon request. The vector set will soon be available from AddGene. [Maury et al, 2016]

CRISPR-Cas9 vectors for efficient genome editing of laboratory and industrial strains of S. cerevisiae (knock-ins and knock-outs) are available from AddGene. [Order] [Stovicek et al, 2015]

cloning tool

Engineered Saccharomyces cerevisiae strains for production of chemicals

All the published strains are available upon request.
- for production of 3-hydroxypropionic acid from glucose via malonyl-CoA pathway [Kildegaard & Jensen et al, 2016]
- for production of 3-hydroxypropionic acid from xylose [Kildegaard et al, 2015]
- industrial xylose-utilizing S. cerevisiae strains [Stovicek et al, 2015]
- for production of resveratrol via tyrosine ammonia lyase [Li et al, 2015] or via phenylalanine ammonia lyase [Li et al, 2016]
- for production of p-coumaric acid [Rodriguez et al, 2015]
- for production of 3-hydroxypropionic acid from glucose via beta-alanine pathway [Borodina et al, 2015]
- tolerant to 3-hydroxypropionic acid [Kildegaard et al, 2014]


Irina Borodina
Senior Researcher
DTU Biosustain
+45 45 25 80 20